Application of pulsed electric fields for the biocompatible extraction of proteins from the microalga Haematococcus pluvialis

This study aims to employ a pulsed electric field (PEF) treatment for the biocompatible (non-destructive) extraction of proteins from living cells green microalga Haematococcus pluvialis. Using strength 1 kV cm?1, we achieved 10.2 µg protein per mL culture, which corresponded 46% total amount that could be extracted by complete destructive (i.e. grinding biomass with glass beads). We found yield was not improved stronger strengths and dependent on pulse frequency. A biocompatibility index (BI) defined as relative abundance remained alive after PEF treatment. relied measurements several physiological parameters It at cm?1 cultures recovered 72 h. Therefore, these conditions constituted good compromise between efficiency culture survival. To characterize further molecular level, mass spectrometry-based proteomics analyses PEF-prepared extracts used. led identification 52 electro-extracted proteins. Of these, only 16 were identified when 0.5 cm?1. They belong core metabolism, stress response cell movement. Unassigned also extracted. Their implications possible utilization in food alimentary complements are discussed.